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Medical Definition of Gt-ag rule
1. This is the observation that all introns in DNA begin with the nucleotides of GT(guanine, thymine) and end with the nucleotides AG (adenine, guanine). When the DNA is transcribed into RNA, the introns are removed from the RNA by a mechanism which recognises these beginning and ending nucleotides - in the RNA they would be CU (cytosine, uracil) and AC (adenine, cytosine). (09 Oct 1997)