2. Verb. To perform such an analysis ¹
¹ Source: wiktionary.com
Definition of Immunoblot
1. [n -S]
Medical Definition of Immunoblot
1. Commonly referred to as Western blot because of the similarity to the procedures described above, is used to detect antibodies to specific epitopes of electrophoretically separated subspecies of antigens. Electrophoresis of antigenic material yields separation of the antigenic components by molecular weight. Blotting of the separated antigen to nitrocellulose, retaining the electrophoretic position, and reacting it with patient specimen will result in the binding of specific antibodies, if present, to each antigenic "band". Electrophoresis of known molecular weight standards allows for the determination of the molecular weight of each antigenic band to which antibodies may be produced. These antibodies are then detected using EIA reactions which characterise antibody specificity. This technique is often used to confirm the specificity of antibodies which are detected by ELISA screening procedures. (05 Mar 2000)
Lexicographical Neighbors of Immunoblot
Literary usage of Immunoblot
Below you will find example usage of this term as found in modern and/or classical literature:
1. Screening for Infectious Diseases Among Substance Abusers: A Treatment by DIANE Publishing Company (1994)
"... the supplemental anti-HCV immunoblot system (Matrix HCV, Abbott); and the
recombinant immunoblot assay system (RIBA-1, Ortho Diagnostics). ..."
2. A Lifelong Passion: Nicholas and Alexandra: Their Own Story by Andrei Maylunas (2005)
"immunoblot analysis of the soluble extract of soybean root tips with mono- specific
anti-PCNA/cyclin identified an immunoreactive protein with an apparent ..."
3. Reviews in Environmental Health (1998): Toxicological Defense Mechanics edited by Gary E. R. Hook, George W. Lucier (2000)
"... testing. normal tissues, and the total amount of p21 that is detected by
immunoblot analysis is 2 to 3 times higher in Tg mice dian in non-Tg mice (13). ..."
4. Oxygen/Nitrogen Radicals and Cellular Injury edited by Kenneth B. Adler, Robert D. Devlin, Val Vallyathan (2000)
"... and express NOS2 mRNA (reverse transcriptase—polymerase chain reaction) and
protein (immunoblot and immunohistology). The NOS2 was associated primarily ..."
5. Manual of Microbiologic Monitoring of Laboratory Animals edited by Kim Waggie (1994)
"... Günther R. Enzyme-linked immunosorbent assay and immunoblot analysis of the
immunoglobulin G response to whole-cell and lipooligosaccharide antigens of ..."
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