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Medical Definition of Diffusion method
1. A method for the study of bacterial enzymes in which agar is mixed with the material (e.g., starch or milk) which is to serve as an indicator of the enzyme action and is inoculated and plated; if the bacteria produce enzymes digesting the admixed material, there will be a zone of clearing in the medium about each colony. Synonym: diffusion method. (05 Mar 2000)