Medical Definition of Blue white colour selection
1.
Method for identifying bacterial clones containing plasmids with inserts. Many modern vectors have their polycloning site within a part of the LacZ gene encoding _ galactosidase, which provides _ complementation in an appropriate mutant E. Coli strain. This means that a re ligated (empty) vector will produce blue colonies when grown on plates containing IPTG and X gal, but colonies with a substantial insert in their plasmid's polycloning site are unable to produce functional _ galactosidase and so produce white colonies.
(16 Dec 1997)
Lexicographical Neighbors of Blue White Colour Selection
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