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Definition of Biotinylation
1. Noun. (biochemistry) The attachment of a biotin residue to a biological macromolecule in order to label it. ¹
¹ Source: wiktionary.com
Medical Definition of Biotinylation
1. A technique whereby biotinyl groups are incorporated into molecules, either that catalyzed by holocarboxylase synthetase during enzyme biosynthesis or that undertaken in vitro to visualise specific substrates by incubating them with biotin-labelled probes and avidin or streptavidin that has been linked to any of a variety of substances amenable to biochemical assay. Biotinylated dutp is an alternative method to radioactive DNA labelling in vitro. (12 Dec 1998)