Medical Definition of Replica plating

1. Technique for testing the genetic characteristics of bacterial colonies. A dilute suspension of bacteria is first spread, in a petri dish, on agar containing a medium expected to support the growth of all bacteria, the master plate. Each bacterial cell in the suspension is expected to give rise to a colony. A sterile velvet pad, the same size as the petri dish, is then pressed onto it, picking up a sample of each colony. The bacteria can then be stamped onto new sterile petri dishes, plates, in the identical arrangement. The media in the new plates can be made up to lack specific nutritional requirements or to contain antibiotics. Thus colonies can be identified that cannot grow without specific nutrients or that are antibiotic resistant and cells with mutations in particular genes can be isolated. This entry appears with permission from the Dictionary of Cell and Molecular Biology (11 Mar 2008)



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Lexicographical Neighbors of Replica Plating

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replevied
replevies
replevin
replevined
replevinger
replevingers
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replevins
replevisable
replevy
replevying
replica
replica methods
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replica plating (current term)
replica techniques
replicabilities
replicability
replicable
replicant
replicants
replicas
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replicases
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replicated
replicated worker
replicated workers
replicates

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