Medical Definition of Hybridisation stringency
1.
The percentage of nucleotides which must match on two unrelated single-stranded nucleic acid molecules before they will base pair with each other to form a duplex, given a certain set of physical and chemical conditions.
The hybridisation stringency is used to determine when a hybridisation probe and a target nucleic acid will come together, and can be set by the researcher by varying the conditions. In general, if the percentage of matching nucleotides is lower than 70 percent, the two single-stranded nucleic acid molecules are considered nonhomologous and any hybridisation is considered nonstringent.
(13 Oct 1997)
Lexicographical Neighbors of Hybridisation Stringency
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