Medical Definition of Dideoxy sequencing

1. The most popular method of DNA sequence determination (c.f. Maxam Gilbert sequencing). Starting with single stranded template DNA, a short complementary primer is annealed and extended by a DNA polymerase. The reaction is split into 4 tubes (called A, C, G or T) each containing a low concentration of the indicated dideoxy nucleotide, in addition to the normal deoxynucleotides. Dideoxynucleotides, once incorporated, block further chain extension and so each tube accumulates a mixture of chains of lengths determined by the template sequence. The 4 reactions are denatured and run out on an acrylamide sequencing gel in neighbouring lanes and the sequence read up the gel according to the order of the bands. This entry appears with permission from the Dictionary of Cell and Molecular Biology (11 Mar 2008)



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Lexicographical Neighbors of Dideoxy Sequencing

didee
didees
didelphia
didelphian
didelphic
didelphid
didelphids
didelphine
didelphous
didelphyc
didemnaketal
didemnaketals
didendron
dideoxy
dideoxy procedure
dideoxy sequencing (current term)
dideoxyadenosine
dideoxycytidine
dideoxyinosine
dideoxynucleosides
dideoxynucleotide
dideoxynucleotides
dideoxyribonucleoside
dideoxyribonucleosides
dideoxysugar
dideoxysugars
dideoxythymidine
didesmethyldoxylamine
didgeridoo
didgeridooer

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