Medical Definition of Sanger Coulson method

1. The most popular method of DNA sequence determination (c.f. Maxam Gilbert sequencing). Starting with single stranded template DNA, a short complementary primer is annealed and extended by a DNA polymerase. The reaction is split into 4 tubes (called A, C, G or T) each containing a low concentration of the indicated dideoxy nucleotide, in addition to the normal deoxynucleotides. Dideoxynucleotides, once incorporated, block further chain extension and so each tube accumulates a mixture of chains of lengths determined by the template sequence. The 4 reactions are denatured and run out on an acrylamide sequencing gel in neighbouring lanes and the sequence read up the gel according to the order of the bands. This entry appears with permission from the Dictionary of Cell and Molecular Biology (11 Mar 2008)

Lexicographical Neighbors of Sanger Coulson Method

Sandstrom's bodies
Sandwich
Sandwich Islander
Sandwich Islands
Sandwich Tern
Sandwich Terns
Sandwicher
Sandwichers
Sandy
Sanfilippo's syndrome
Sanfillipo syndrome
Sangamon
Sangay
Sanger
Sanger's reagent
Sanger Coulson method (current term)
Sanger method
Sangho
Sango
Sangraal
Sangu
Sanguinaria canadensis
Sanguisuga
Sanhedrin
Sanhedrins
Sanhedrist
Sanhedrists
Sanhita
Sanhitas
Sanicula

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